specimen preparation
specimen preparation for optical microscope, wet mount and hanging drops mount, fix mount, staining, base dyes and positive staining, acidic dyes and negative staining, simple stains, differential stain, structural stain, staining examples, culturing of microbes, inoculation, isolation, incubation, inspection, information gathering, identification, isolation techniques, ways to identify microbes, media, classification of media according to three properties, physical state, chemical composition, functional type, broth, agar, most commonly used media, nutrient broth and nutrient agar, chemical content of media, examples of enriched media, selective and differentiated media, techniques in cultivatiing and identifying animal viruses, methods of growing animal viruses
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Time limit: 10 minutes
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Question 1
What is the main purpose of culturing microbes?
Explanation
The main purpose of culturing microbes is to increase their population for further study and experimentation.
Question 2
What is a wet mount used for in microscopy?
Explanation
A wet mount is a technique used to prepare specimens for viewing under a microscope, allowing observation of live organisms and their movement.
Question 3
What type of staining uses basic dyes to color specimens?
Explanation
Positive staining uses basic dyes to color specimens, which helps in visualizing cellular structures.
Question 4
Which staining technique uses acidic dyes?
Explanation
Negative staining uses acidic dyes that repel the cell walls, providing a contrasting background to help visualize the cells.
Question 5
What does the term 'inoculation' refer to in microbiology?
Explanation
Inoculation refers to the process of introducing microbes into a culture medium to grow them.
Question 6
Which of the following is a type of nutrient media?
Explanation
Agar is a type of nutrient medium commonly used to culture various microorganisms.
Question 7
What is a common characteristic of enriched media?
Explanation
Enriched media are designed to support the growth of fastidious organisms that require specific nutrients.
Question 8
What is the primary function of differential staining?
Explanation
Differential staining is used to differentiate between different types of cells based on their staining properties.
Question 9
What is the first step in the microbial investigation process?
Explanation
Inoculation is the first step in the microbial investigation process, where microbes are introduced into a culture medium.
Question 10
Which of the following is an example of positive staining?
Explanation
A simple stain is a type of positive staining technique that uses a single dye to color cells.
Question 11
What describes a selective media?
Explanation
Selective media are designed to inhibit the growth of unwanted organisms while allowing the growth of specific microbes.
Question 12
What does isolation techniques refer to in microbiology?
Explanation
Isolation techniques involve separating individual microbes from a mixed population to study them in detail.
Question 13
What is the function of incubation in microbial culture?
Explanation
Incubation provides the necessary conditions for microorganisms to grow and reproduce.
Question 14
Which type of media is commonly used for growing bacteria?
Explanation
Nutrient agar is a widely used medium for cultivating microorganisms, particularly bacteria.
Question 15
What is the end goal of inspection in microbial studies?
Explanation
The goal of inspection is to gather information about the cultured microorganisms for classification and identification.
Question 16
How are animal viruses commonly cultured?
Explanation
Animal viruses are typically cultured inside living cells, as they require host cellular machinery to reproduce.
Question 17
Which of the following best categorizes media based on its physical state?
Explanation
Media can be categorized based on their physical state into liquid and solid forms.
Question 18
What is the main purpose of information gathering in microbiology?
Explanation
Information gathering is crucial for identifying the microorganism and understanding its characteristics.
Question 19
What is the purpose of a wet mount preparation in microscopy?
Explanation
A wet mount preparation allows the observer to view living specimens in their natural environment, providing insights into their movement and behavior.
Question 20
Which type of media is used to grow microorganisms in a liquid form?
Explanation
Broth media are specifically designed for cultivating microorganisms in a liquid state, allowing for growth and observation of microbial characteristics.