General laboratory techniques

Quiz Type

Practice

Subject

Biomedical Science

Level

Level 200

Questions

10 questions

Created By

Megan Ashong - Lamptey

Quick Rules

Time limit: 50 minutes
Multiple attempts are not allowed
All questions must be answered to submit

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Question 1 of 10

Question 1

Basic multiple choice Remembering

What is the primary purpose of a laboratory technique?

To conduct experiments and analyze results

Correct

To create artistic displays of scientific data

To eliminate the need for scientific research

To replace the need for scientists

Explanation

Laboratory techniques are designed to conduct experiments and analyze data to further scientific understanding.

Question 2

Basic multiple choice Remembering

Which of the following is a common laboratory technique?

Titration

Correct

Filming

Painting

Cooking

Explanation

Titration is a widely used laboratory technique for determining the concentration of a substance in a solution.

Question 3

Basic multiple choice Understanding

Why is it important to follow safety protocols in a laboratory?

To prevent accidents and ensure safety

Correct

To avoid losing lab equipment

To make the lab look organized

To impress others with your knowledge

Explanation

Following safety protocols is crucial to prevent accidents and ensure a safe environment for all laboratory workers.

Question 4

Basic multiple choice Remembering

What does a pipette do in a laboratory setting?

Measures and transfers liquid

Correct

Heats substances

Stores solid materials

Scribes notes automatically

Explanation

A pipette is a laboratory instrument used to measure and transfer specific volumes of liquid accurately.

Question 5

Basic multiple choice Understanding

What is the role of a microscope in laboratory techniques?

To magnify small objects

Correct

To record data digitally

To create chemical reactions

To cool down substances

Explanation

A microscope is used to magnify small objects, allowing scientists to see details that are not visible to the naked eye.

Question 6

Complex multiple choice Understanding

A laboratory technician is conducting experiments on a new drug compound. They need to determine the optimal temperature for a series of reactions. Given that temperature can affect reaction rates and yields, which general laboratory technique should they prioritize when collecting data for their analysis?

Conducting a titration to measure concentrations

Utilizing a spectrophotometer to assess absorbance changes

Performing a temperature gradient experiment

Correct

Implementing a chromatography process to separate components

Explanation

The correct approach is to perform a temperature gradient experiment, as it allows the technician to systematically analyze how varying temperatures affect reaction rates and yields. While titrations, spectrophotometry, and chromatography are important techniques, they focus on different aspects of analysis rather than directly evaluating temperature effects.

Question 7

Complex multiple choice Analyzing

During a routine laboratory procedure, a scientist mistakenly mixes incompatible chemicals in a reaction setup. To avoid similar incidents in the future, they decide to improve their laboratory techniques. Which of the following strategies should they implement to enhance safety and accuracy in future experiments?

Label all chemicals clearly and conduct experiments without reviewing safety data sheets

Organize a well-documented standard operating procedure (SOP) for chemical handling

Correct

Only rely on personal experience rather than consulting scientific literature

Use visual aids that misrepresent chemical compatibility

Explanation

Establishing a well-documented standard operating procedure (SOP) for chemical handling is the most effective strategy to ensure safety and accuracy in future experiments. Clear labeling and reviewing safety data sheets are part of this process, while relying solely on personal experience or using misleading visual aids can lead to dangerous situations.

Question 8

Case based multiple choice Understanding

[Case Scenario] A research team is working on a project to evaluate the purity of a new compound they have synthesized in the laboratory. They have performed several purification techniques including recrystallization and chromatography. After obtaining a sample, they analyze it using thin-layer chromatography (TLC) to determine the presence of any impurities. The results show multiple spots instead of a single spot, indicating that the sample isn't pure. Question: Based on the laboratory techniques used, what could be the most plausible explanation for the multiple spots observed in the TLC analysis?

Analysis A: The compound has been synthesized incorrectly.

Analysis B: The recrystallization process failed to adequately remove impurities.

Correct

Analysis C: The TLC plate was contaminated.

Analysis D: The development solvent was too polar.

Explanation

In this scenario, the presence of multiple spots in the TLC indicates that the purification method, possibly recrystallization, was not effective enough to fully separate the desired compound from impurities, leading to the observed outcome in the TLC analysis.

Question 9

Case based multiple choice Understanding

[Case Scenario] During a laboratory practical, a student is directed to perform a titration to determine the concentration of an acetic acid solution. The student accurately prepares the titration setup, using a burette filled with a sodium hydroxide solution of known concentration and phenolphthalein as the indicator. After carefully adding the sodium hydroxide solution dropwise, the student observes a color change from colorless to pink, indicating the endpoint of the reaction. Question: What conclusion can the student draw from the observation of the color change during the titration?

Analysis A: The acetic acid solution has reached its maximum volume.

Analysis B: The sodium hydroxide has completely reacted with the acetic acid.

Correct

Analysis C: The acetic acid is completely neutralized.

Analysis D: The phenolphthalein indicator was improperly used.

Explanation

The color change from colorless to pink signifies that the acetic acid has reached equivalence with sodium hydroxide; thus, the result of the titration reflects the completion of the reaction.

Question 10

Case based multiple choice Understanding

[Case Scenario] A laboratory technician is performing a series of experiments using spectrophotometry to measure the concentration of a colored solution. The technician prepares a calibration curve using a set of standard solutions with known concentrations and measures their absorbance values. The resulting plot of absorbance versus concentration results in a linear relationship, which the technician uses to estimate the concentration of an unknown sample. The absorbance of the unknown sample falls along the line of the calibration curve. Question: What inference can the technician make about the concentration of the unknown sample based on the relationship established in the calibration curve?

Analysis A: The unknown sample has a concentration significantly higher than the highest standard.

Analysis B: The unknown sample has a concentration equal to one of the standard concentrations.

Analysis C: The concentration of the unknown sample can be determined based on its absorbance value.

Correct

Analysis D: The linearity of the calibration curve indicates that no other variables affect the absorbance.

Explanation

The linear relationship of the calibration curve allows for the estimation of the unknown sample's concentration based on its observable absorbance, confirming the utility of spectrophotometry in quantitative analysis.