GEMP - Biological media
Biological Media and Its Preparation Introduction Biological media is a critical component in microbiology and biomedical fields, serving as a nutrient-rich environment that supports the growth and maintenance of microorganisms, cells, and tissues in laboratory settings. Understanding the preparation and application of biological media is vital for medical students as it forms the foundation ...
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Time limit: 5 minutes
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Question 1
Multiple choiceWhich of the following components is NOT typically found in biological media?
Explanation
Heavy metals are not standard components of biological media. Common components include carbohydrates, proteins, and vitamins.
Question 2
Multiple choiceWhat is the primary purpose of biological media in microbiology?
Explanation
Biological media provide a nutrient-rich environment necessary for the growth and maintenance of microorganisms, which is crucial in microbiology and biomedical fields.
Question 3
Multiple choiceWhat role do buffering agents play in biological media?
Explanation
Buffering agents are crucial for maintaining an optimal pH, ensuring that organisms can thrive without the adverse effects of pH changes.
Question 4
Multiple choiceWhich of the following media is specifically formulated for the cultivation of fungi?
Explanation
Sabouraud Dextrose Agar is designed for cultivating fungi, particularly yeasts and molds, due to its increased glucose concentration.
Question 5
Multiple choiceWhich of the following statements about selective and differential media is true?
Explanation
Selective media have specific inhibitors that restrict the growth of certain organisms, whereas differential media include indicators to distinguish different species.
Question 6
Multiple choiceWhich type of biological medium is primarily used for isolating and counting microbial colonies?
Explanation
Solid media are specifically used for isolating and counting colonies, allowing for easy visualization of distinct colonies.
Question 7
Multiple choiceWhat is the primary function of peptones in biological media?
Explanation
Peptones are derived from protein hydrolysis and are rich in amino acids, serving as an important nutrient for growth.
Question 8
Multiple choiceWhy is it a misconception that all biological media are interchangeable?
Explanation
Different microorganisms have unique nutritional requirements and environmental preferences; using inappropriate media can hinder growth or lead to misidentification.
Question 9
Multiple choiceIn preparing Nutrient Agar, how much beef extract is used per liter?
Explanation
Nutrient Agar formulation includes 3g of beef extract per liter, providing necessary nutrients for microbial growth.
Question 10
Multiple choiceWhat is the key purpose of using agar in biological media?
Explanation
Agar is used to solidify biological media, providing a stable surface for the growth of colonies of microorganisms.